ELISA Kits are useful in many different scientific areas, such as peptide synthesis, to accurately find and measure antibodies or antigens. This flexibility makes them a critical tool for research and development, helping scientists understand the complexities of biological systems.
The ELISA Antibody expert can be used for the detection of many targets including hormones, antibodies, cytokines, tumour markers, infectious diseases and diabetes. Various assay formats can be used including competitive and sandwich ELISAs with both 96-well and 384-well microplates available. Each ELISA kit includes pre-coated plates with capture/detection antibodies, standards and accessory reagents, and each goes through fit-for-purpose validation for reliability and stability.
Custom Monoclonal Antibodies: Tailored Solutions for Your Research
An ELISA assay measures the concentration of an analyte in a sample such as serum, plasma, cell culture supernatants, tissue lysates, saliva or urine. The assay consists of a capture antibody which binds to the target analyte, followed by a conjugated detection antibody which recognises a different epitope on the same antigen. Addition of a substrate produces a readout in the form of an absorbance (color), fluorescence or electrochemical signal and is proportional to the amount of analyte bound by the detection antibody.
ELISAs are often a screening tool before more in-depth scientific testing takes place, making it important that the technique is performed correctly to produce accurate results. This is important as incorrect use of the ELISA kit can lead to false positive and false negative results, which can have serious health implications.